Sondy Molecular Beacon

Molecular Beacon Probes

Nazwa

Ilość dostarczona

Ilość reakcji

Molecular Beacon for the EVAluation.

300 pmol

100

Molecular Beacon Probe

1.0 OD

1 000

Molecular Beacon Probe  2.0 OD  2 000
Molecular Beacon Probe  5.0 OD  5 000
Molecular Beacon Probe  10 OD  10 000
Molecular Beacon Probe  30 OD  30 000
Additional nucleotides for probes longer than 30 bases

Molecular Beacon Probes with 5'-reporter (FAM, TET, HEX, YAK, Cy3, Cy5) and terminale quencher (Dabcyl, TAMRA, BHQ1™, BHQ2™).
The quencher TAMRA (rhodamin) is by itself a fluorophore, absorved energy is emitted as fluorescence. 'Dark Quencher' do not emit light and therefore, do not interfere with the measurement. 'Dark Quencher' Dabcyl [DiAzoBenzyl, = Methylorange].
Dabcyl and BHQ1™ can be used for dyes with an emmission wave length of up to 600 nm. BHQ1™ and BHQ2™ cover wave lenghts up to 700 nm.

Fluorescence-increase determination in a melting curve(LightCycler®)
Molecular Beacon Validated Probe (MVP). Synthesis of a target sequence, determination and comparison of fluorescence in its presence and melting curve analysis (LightCycler®).

Molecular Beacon Probes with 5'-reporter (JOE, ROX, TEXAS, Bodipy, MegaStokes) and terminal quencher (Dabcyl, TAMRA, BHQ1™, BHQ2™).

Nazwa

Ilość dostarczona

Ilość reakcji

Molecular Beacon for the EVAluation.

300 pmol

100

Molecular Beacon Probe

1.0 OD

2 000

Molecular Beacon Probe  2.0 OD  2 000
Molecular Beacon Probe  5.0 OD  5 000
Molecular Beacon Probe  10 OD  10 000
Molecular Beacon Probe  30 OD  30 000
Additional nucleotides for probes longer than 30 bases

Fluorescence-increase determination in a melting curve(LightCycler®)
Molecular Beacon Validated Probe (MVP). Synthesis of a target sequence, determination and comparison of fluorescence in its presence and melting curve analysis (LightCycler®).