Sondy Molecular Beacon

Molecular Beacon Probes with 5'-reporter (FAM, TET, HEX, YAK, Cy3, Cy5) and terminale quencher (Dabcyl, TAMRA, BHQ1™, BHQ2™).
The quencher TAMRA (rhodamin) is by itself a fluorophore, absorved energy is emitted as fluorescence. 'Dark Quencher' do not emit light and therefore, do not interfere with the measurement. 'Dark Quencher' Dabcyl [DiAzoBenzyl, = Methylorange].
Dabcyl and BHQ1™ can be used for dyes with an emmission wave length of up to 600 nm. BHQ1™ and BHQ2™ cover wave lenghts up to 700 nm.

Fluorescence-increase determination in a melting curve(LightCycler®)
Molecular Beacon Validated Probe (MVP). Synthesis of a target sequence, determination and comparison of fluorescence in its presence and melting curve analysis (LightCycler®).

Molecular Beacon Probes with 5'-reporter (JOE, ROX, TEXAS, Bodipy, MegaStokes) and terminal quencher (Dabcyl, TAMRA, BHQ1™, BHQ2™).
Nazwa	Ilość dostarczona	Ilość reakcji
Molecular Beacon for the EVAluation.	300 pmol	100
Molecular Beacon Probe	1.0 OD	2 000
Molecular Beacon Probe	2.0 OD	2 000
Molecular Beacon Probe	5.0 OD	5 000
Molecular Beacon Probe	10 OD	10 000
Molecular Beacon Probe	30 OD	30 000
Additional nucleotides for probes longer than 30 bases

Fluorescence-increase determination in a melting curve(LightCycler®)
Molecular Beacon Validated Probe (MVP). Synthesis of a target sequence, determination and comparison of fluorescence in its presence and melting curve analysis (LightCycler®).